Microorganisms are found throughout the environment: in the air and water; on the surface of any object such as clothes, walls, furniture; in soil and dust; and on and in our own bodies skin and mucous membranes. The results of the exercise or experiment are what are being showcased, not the writing. Once all these tests are performed, the unknown bacteria in this lab will be one of the following: Escherichia coli, Enterobacter aerogenes, Proteus mirabilis, Klebsiella pneumoniae, or Salmonella typhimurium. The lid was then put back on the plates and the loop was again sterilized by burning until glowing bright red on the Bunsen burner. These plates were incubated for two days to allow the bacteria to grow. The first test used was a gram stain.
The T- streak isolation method will make sure there is no contamination in the unknown. A gram stain was taken from a sample of the bacteria growing on the nutrient agar. This was isolated and a Gram stain was performed. The streak plate method utilizes an inoculating loop, sterilized by a Bunsen burner provided, to decrease chance of contamination on the nutrient agar plate 2. The next biochemical test that was performed was a H2S test.
The methods used for The colonies were smooth, translucent, and had a white brownish color. This could have happened when attempting to isolate the bacterium on to two separate plates. When observed, there were both gram-positive rods and gram-negative rods. The finding could have been incorrect because the clearing around the bacterium might not have been as clear for the tester to notice. Multiply this by 6 to get the number of beats in 60 seconds this your pulse. This is deduced by the addition of Reagent A and Reagent B, and the observation of the color change thereafter. A negative urea test removed S.
An integral part of any medical treatment is to be able to recognize and. Free Essay Crowder-Gross Lab 2 Report 3-In this activity you learn open Server Manager and then see what all the different things it can do. Prepare an area in which the student is able to keep his beakers, and arrive after 1 hr to record the results. The starch test was used to identify which bacterium can produce the enzyme amylase. This was performed to ensure that the Gram positive bacteria were well isolated from the Gram negative.
For this reason, it is important to test and observe unknown organisms in the lab to continually improve the health and well being of society. Scientific writing is written differently from other types of writing. The methods and tests learned through out the semester were used on this test tube in order to identify the two unknown microorganism; one a Gram positive and the other a Gram negative. Example of a Microbiology Unknown Lab Report by Taylor Autry Introduction In this paper I will discuss the processes of how I came to find my two unknown bacteria. It is of great importance to recognize and identify the different instruments and laboratory tools, because in this way will we be able to use them properly and also to call them by name and know why. One of the unknowns found, Escherichia coli, is a very vast and diverse species of bacteria. Included are the materials and methods used to identify the gram positive and gram negative bacteria and methods which also differentiate between microbes of each group.
Koneman's Color Atlas and Textbook of Diagnostic Microbiology. Any other color produced other than red means the organism is not capable of mixed acid fermentation. There are four safety equipment items that a lab should have. Pathogenic bacteria are bacteria that cause bacterial infection. This negative result indicated that unknown bacteria 25 did not produce any acetoin and was not a fermenter of 2,3-buteandiol. A gram stain reaction test is used to differentiate between two bacterial species.
To obtain the identification of this unknown bacterium, several biochemical tests needed to be performed in order to prescribe the correct medication to treat and cure the symptoms. After conducting each of these tests, the unknown bacteria number 24 was concluded to be Proteus mirabilis. The first step to figuring out the unknowns, was to separate the two bacteria. They are remarkably adaptable to diverse environmental conditions and are found in bodies of all living organisms and on all parts of the earth. The purpose of scientific writing is not to entertain, but to inform. Alt 5 was given as a substitute for a gram-negative bacterium, due to no growth in original test tube 122.
Words: 4133 - Pages: 17. A Gram stain discovered that the bacteria were rod shaped. This could cause painful infections such as urinary tract infections 3. Twenty percent of the human population are carriers of S aureus, which can be found on the skin and inside the nasal passages. Moreover, we made some programs and let the robots memorize it. Bacteria, Enterobacteriaceae, Escherichia coli 1053 Words 4 Pages Identification of Two Unknown Species of Bacteria in Tube 72 Introduction: There are many reasons for knowing the identity of microorganisms.
However, like stated earlier, a problem was faced after the isolation was incubated. Confirming that the unknown was a gram-negative bacterium, the next step. With the disks on the inoculated petri dish, the petri dish must be sealed and placed into an incubator at an exact temperature of 37°C for 24 hours and allow the bacteria to grow. We then put the inoculated plate media into the incubator for the next lab period. To start, the two unknown bacteria had to be successfully isolated. The genus is capitalized but the species is not. An unknown bacterium was handed out by the lab instructor.