A few drops of hydrochloric acid were mixed in the beaker that was containing 50% solution of hydrogen peroxide. Figure 2 shows the reaction rates of the different pH levels. Took round filter paper and made several holes in it with the use of sharp needle. This lab focused on how pH levels affect the function of enzymes. The color was observed with the passage of time to conclude whether amylase activity was present or not. Repeat the steps each time, for every buffer solution as well as distilled water.
To observe this catalase activity I used small amounts of liver samples slightly smaller than the size of a pea to see the reaction when hydrogen peroxide is added to it. Part B - What Tissues Contain Catalase? In short, enzymes reduce the energy needed for a reaction to take place, permitting a reaction to take place more easily. Add 10cm3 of buffer solution to the boiling tube using a different syringe. Catalase is present in every plant and animal organ. In conclusion, the reaction chamber placed in the highest temperature water bath produced an average of. All three tubes had maltose present.
As previously stated, enzymes speed up chemical reactions through the lowering of activation energy. This is because enzymes function best at an optimal pH, which in our case is around 7. The enzymes are what assist in making this happen. The enzyme Amylase breaks down the carbohydrate starch into the product maltose, so the amounts of starch were measured after certain times to see how salt percentages and pH levels affected the activity of Amylase. You will be using chicken or beef liver.
That is why it is important to use equal amounts each time, as well as planning out the experiment beforehand. The active site is the only part of an enzyme that actually binds to a substrate, usually a pocket or groove on the surface of the protein. The liver will have a large reaction because the majority of liver is proteins and enzymes which break down the Hydrogen Peroxide. Cut 6 cubes of liver approximately 1cm x 1cm x 1cm 2. Graph your results as substrateconcentration x-axis vs. Add 2 ml of hydrogen peroxide.
After adding Hydrochloric Acid onto the liver the reaction rate seemed to diminish as the acidity increased. Amylase is an type of enzyme. The first characteristic of an enzyme is to increase inside the human body depending on the rate of reaction. The second was 1 mL of starch and 50 µL of water. The uncertainties of my apparatus ±2% for the first 1o ml graduated cylinder and another ±2% for the second one. Potatoes, apples and bananas contain catechol oxidase which acts on colorless catechol and converts it to brown-colored melanin. Next the substrate is changed to its product, the product is released and the enzymes active site is ready and waiting for another molecule of substrate.
How do these reactions occur? The above activity was related to the enzyme. Download file to see next pages Read More. Put a piece of liver into the bottom of a clean test tube and cover it with a small amount of water. Placed the same filter paper in hydrogen peroxide and noted the time until the reaction completes. Maltose was shown to be present in all three test tubes.
This step was repeated every minute to test the presence of starch. The test tubes were immersed in 4 water baths that were at the temperature labeled on the test tubes. What effect does this have on its properties? Four test tubes were filled with 1 mL of pH 4, 5, 6 and 7 starch solutions respectively. Specifically, discuss whether or not your hypotheses were supported. Using the forceps take one cube of liver and place it in the measuring cylinder.
Using a syringe, measure out 10cm3 of starch solution into a boiling tube. Materials andMethods: At your lab table, youwill find everything you need to conduct today's experiments. The 37°C completed the reaction the quickest. Each article reference should be given as in the following example: Alfano J. Use the results ofthe trial from part A, step1 the catalase reaction with 3 disks atroom temperature for the Room temperature data point requested onthe class spreadsheet.